HPV Assay:Article Title: Accuracy of self-collection in human papillomavirus-based cervical screening: An evidence-based review
Article Snippet: .. The Copan FLOQSwab® (Copan, Brescia, Italy) and Rovers Evalyn® Brush (Rovers Medical Devices, Lekstraat, The Netherlands) are the devices currently validated by HPV assay manufacturers, but others might be independently validated by pathology laboratories. ..
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Article Title: Development of a Rapid Automated Point-of-Care Test for Mycobacterium tuberculosis Detection from Tongue Swabs and Sputum Specimens on the DASH® Rapid PCR System
Article Snippet: Ten positive and 20 negative tongue swab specimens were provided by the Theron lab. Tongue swabs were collected using the Copan FLOQSwab (520CS01) from a cohort of adults self-reporting with presumptive pulmonary TB symptoms meeting WHO criteria (at least one symptom for people living with (PLHIV), ≥2 for those without HIV) and recruited at clinics in Cape Town, South Africa.
Article Title: Development of a Rapid Automated Point-of-Care Test for Mycobacterium tuberculosis Detection from Tongue Swabs and Sputum Specimens on the DASH® Rapid PCR System
Article Snippet: The swab pairs were either Copan FLOQSwab (520CS01) or Steripack swab (60564RevC) stored dry at −80°C.
Biomarker Discovery:Article Title: Accuracy of self-collection in human papillomavirus-based cervical screening: An evidence-based review
Article Snippet: .. For example, the swab cannot currently be used during pregnancy when the manufacturer-validated methods (Roche and BD) are used, as these manufacturers have based their restrictions on the Copan FLOQSwab instructions for use.11 Copan currently states that vaginal self-collection should not be performed during pregnancy; however, some laboratories have performed in-house validation for using the swab during pregnancy, provided the swab is transported dry. ..
Adhesive:Article Title: Touch DNA in forensic science: a comprehensive overview and current status of the research
Article Snippet: Researchers conducted a comparative analysis of various techniques, including dry and wet cotton swabs with three different swabbing motions, the double-swab (wet-dry) method, wet and dry Copan FLOQSwabsTM, and Scene Safe FASTTM minitapes. .. An analysis was conducted to identify the most appropriate technique for collecting accurate STR profiles from touch DNA samples found on skin surfaces “Single-swabbing, double-swabbing, adhesive tapes” DNA Extraction- “Chelex extraction” The findings suggest that the various DNA collecting methods, such as the COPAN FLOQSwab, the double-swab approach, and normal swabbing with standard cotton swabs, yielded comparable results Quantification- “QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific)” Amplification- “AmpFlSTR® NGM SElectTM PCR Amplification Kit (Thermo Fisher Scientific)” Table 1 (continued) .. Nationality Experimental model Sampling method Kits used Important findings Kanokwongnuwut et al., 2021, Australia A study was carried out to determine the minimum number of stained cells required to generate informative STR DNA profiles from touch samples (corneocytes) and saliva samples (buccal cells) processed through different methods “Double Swabbing, adhesive tapes” DNA Extraction- “DNA IQTM System (Promega, Madison, WI, USA)” Tape lifting is only effective in gathering coenocytes, which have a lower amount of DNA, and is not suitable for collecting cell-free DNA from smooth surfaces Quantification- “Investigator® Quantiplex Pro RGQ kit (QIAGEN, VIC, AUS)” Amplification- “Rotor-Gene Q (QIAGEN)” Al-Snan 2021, Bahrain Touch DNA was successfully extracted from evidence contaminated with varying amounts of RDX-C4 without causing DNA degradation or amplification inhibition “Double Swabbing, adhesive tapes, direct cutting” DNA Extraction- “Magnetic beads chemistry (EZ1 Advanced XL, Qiagen, Germany) and AutoMate Express DNA Extraction System, Thermo Fisher Scientific, Inc., Waltham, MA, USA)” Proper handling of RDX-C4 samples is essential.
DNA Extraction:Article Title: Touch DNA in forensic science: a comprehensive overview and current status of the research
Article Snippet: Researchers conducted a comparative analysis of various techniques, including dry and wet cotton swabs with three different swabbing motions, the double-swab (wet-dry) method, wet and dry Copan FLOQSwabsTM, and Scene Safe FASTTM minitapes. .. An analysis was conducted to identify the most appropriate technique for collecting accurate STR profiles from touch DNA samples found on skin surfaces “Single-swabbing, double-swabbing, adhesive tapes” DNA Extraction- “Chelex extraction” The findings suggest that the various DNA collecting methods, such as the COPAN FLOQSwab, the double-swab approach, and normal swabbing with standard cotton swabs, yielded comparable results Quantification- “QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific)” Amplification- “AmpFlSTR® NGM SElectTM PCR Amplification Kit (Thermo Fisher Scientific)” Table 1 (continued) .. Nationality Experimental model Sampling method Kits used Important findings Kanokwongnuwut et al., 2021, Australia A study was carried out to determine the minimum number of stained cells required to generate informative STR DNA profiles from touch samples (corneocytes) and saliva samples (buccal cells) processed through different methods “Double Swabbing, adhesive tapes” DNA Extraction- “DNA IQTM System (Promega, Madison, WI, USA)” Tape lifting is only effective in gathering coenocytes, which have a lower amount of DNA, and is not suitable for collecting cell-free DNA from smooth surfaces Quantification- “Investigator® Quantiplex Pro RGQ kit (QIAGEN, VIC, AUS)” Amplification- “Rotor-Gene Q (QIAGEN)” Al-Snan 2021, Bahrain Touch DNA was successfully extracted from evidence contaminated with varying amounts of RDX-C4 without causing DNA degradation or amplification inhibition “Double Swabbing, adhesive tapes, direct cutting” DNA Extraction- “Magnetic beads chemistry (EZ1 Advanced XL, Qiagen, Germany) and AutoMate Express DNA Extraction System, Thermo Fisher Scientific, Inc., Waltham, MA, USA)” Proper handling of RDX-C4 samples is essential.
Extraction:Article Title: Touch DNA in forensic science: a comprehensive overview and current status of the research
Article Snippet: Researchers conducted a comparative analysis of various techniques, including dry and wet cotton swabs with three different swabbing motions, the double-swab (wet-dry) method, wet and dry Copan FLOQSwabsTM, and Scene Safe FASTTM minitapes. .. An analysis was conducted to identify the most appropriate technique for collecting accurate STR profiles from touch DNA samples found on skin surfaces “Single-swabbing, double-swabbing, adhesive tapes” DNA Extraction- “Chelex extraction” The findings suggest that the various DNA collecting methods, such as the COPAN FLOQSwab, the double-swab approach, and normal swabbing with standard cotton swabs, yielded comparable results Quantification- “QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific)” Amplification- “AmpFlSTR® NGM SElectTM PCR Amplification Kit (Thermo Fisher Scientific)” Table 1 (continued) .. Nationality Experimental model Sampling method Kits used Important findings Kanokwongnuwut et al., 2021, Australia A study was carried out to determine the minimum number of stained cells required to generate informative STR DNA profiles from touch samples (corneocytes) and saliva samples (buccal cells) processed through different methods “Double Swabbing, adhesive tapes” DNA Extraction- “DNA IQTM System (Promega, Madison, WI, USA)” Tape lifting is only effective in gathering coenocytes, which have a lower amount of DNA, and is not suitable for collecting cell-free DNA from smooth surfaces Quantification- “Investigator® Quantiplex Pro RGQ kit (QIAGEN, VIC, AUS)” Amplification- “Rotor-Gene Q (QIAGEN)” Al-Snan 2021, Bahrain Touch DNA was successfully extracted from evidence contaminated with varying amounts of RDX-C4 without causing DNA degradation or amplification inhibition “Double Swabbing, adhesive tapes, direct cutting” DNA Extraction- “Magnetic beads chemistry (EZ1 Advanced XL, Qiagen, Germany) and AutoMate Express DNA Extraction System, Thermo Fisher Scientific, Inc., Waltham, MA, USA)” Proper handling of RDX-C4 samples is essential.
Amplification:Article Title: Touch DNA in forensic science: a comprehensive overview and current status of the research
Article Snippet: Researchers conducted a comparative analysis of various techniques, including dry and wet cotton swabs with three different swabbing motions, the double-swab (wet-dry) method, wet and dry Copan FLOQSwabsTM, and Scene Safe FASTTM minitapes. .. An analysis was conducted to identify the most appropriate technique for collecting accurate STR profiles from touch DNA samples found on skin surfaces “Single-swabbing, double-swabbing, adhesive tapes” DNA Extraction- “Chelex extraction” The findings suggest that the various DNA collecting methods, such as the COPAN FLOQSwab, the double-swab approach, and normal swabbing with standard cotton swabs, yielded comparable results Quantification- “QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific)” Amplification- “AmpFlSTR® NGM SElectTM PCR Amplification Kit (Thermo Fisher Scientific)” Table 1 (continued) .. Nationality Experimental model Sampling method Kits used Important findings Kanokwongnuwut et al., 2021, Australia A study was carried out to determine the minimum number of stained cells required to generate informative STR DNA profiles from touch samples (corneocytes) and saliva samples (buccal cells) processed through different methods “Double Swabbing, adhesive tapes” DNA Extraction- “DNA IQTM System (Promega, Madison, WI, USA)” Tape lifting is only effective in gathering coenocytes, which have a lower amount of DNA, and is not suitable for collecting cell-free DNA from smooth surfaces Quantification- “Investigator® Quantiplex Pro RGQ kit (QIAGEN, VIC, AUS)” Amplification- “Rotor-Gene Q (QIAGEN)” Al-Snan 2021, Bahrain Touch DNA was successfully extracted from evidence contaminated with varying amounts of RDX-C4 without causing DNA degradation or amplification inhibition “Double Swabbing, adhesive tapes, direct cutting” DNA Extraction- “Magnetic beads chemistry (EZ1 Advanced XL, Qiagen, Germany) and AutoMate Express DNA Extraction System, Thermo Fisher Scientific, Inc., Waltham, MA, USA)” Proper handling of RDX-C4 samples is essential.
Polymerase Chain Reaction:Article Title: Touch DNA in forensic science: a comprehensive overview and current status of the research
Article Snippet: Researchers conducted a comparative analysis of various techniques, including dry and wet cotton swabs with three different swabbing motions, the double-swab (wet-dry) method, wet and dry Copan FLOQSwabsTM, and Scene Safe FASTTM minitapes. .. An analysis was conducted to identify the most appropriate technique for collecting accurate STR profiles from touch DNA samples found on skin surfaces “Single-swabbing, double-swabbing, adhesive tapes” DNA Extraction- “Chelex extraction” The findings suggest that the various DNA collecting methods, such as the COPAN FLOQSwab, the double-swab approach, and normal swabbing with standard cotton swabs, yielded comparable results Quantification- “QuantifilerTM Trio DNA Quantification Kit (Thermo Fisher Scientific)” Amplification- “AmpFlSTR® NGM SElectTM PCR Amplification Kit (Thermo Fisher Scientific)” Table 1 (continued) .. Nationality Experimental model Sampling method Kits used Important findings Kanokwongnuwut et al., 2021, Australia A study was carried out to determine the minimum number of stained cells required to generate informative STR DNA profiles from touch samples (corneocytes) and saliva samples (buccal cells) processed through different methods “Double Swabbing, adhesive tapes” DNA Extraction- “DNA IQTM System (Promega, Madison, WI, USA)” Tape lifting is only effective in gathering coenocytes, which have a lower amount of DNA, and is not suitable for collecting cell-free DNA from smooth surfaces Quantification- “Investigator® Quantiplex Pro RGQ kit (QIAGEN, VIC, AUS)” Amplification- “Rotor-Gene Q (QIAGEN)” Al-Snan 2021, Bahrain Touch DNA was successfully extracted from evidence contaminated with varying amounts of RDX-C4 without causing DNA degradation or amplification inhibition “Double Swabbing, adhesive tapes, direct cutting” DNA Extraction- “Magnetic beads chemistry (EZ1 Advanced XL, Qiagen, Germany) and AutoMate Express DNA Extraction System, Thermo Fisher Scientific, Inc., Waltham, MA, USA)” Proper handling of RDX-C4 samples is essential.
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